Figure 9
From: NF-kB inhibitor blocks B cell development at two checkpoints
Inhibition of NF-kB binding activity in splenic B cells derived from MIGR1 and IkBa transduced chimeras. Splenic B cells were purified from MIGR1 control or IkBa virus-transduced bone marrow chimeras and treated with medium, anti-CD40, or anti-IgM for 4 hours. Nuclear extracts were prepared from the cells and analyzed by EMSA using 32P-labeled IgkB oligonucleotides as described in Materials and Methods. The transduction efficiency for MIGR1 control or IkBa virus-transduced cells used for this particular experiment is 29% and 15%, respectively. IkBa transduced B cells exhibit decreased NF-kB DNA binding activity, confirming that the IkBa described in the paper is a biological active inhibitor.