Figure 8From: NF-kB inhibitor blocks B cell development at two checkpointsMIGR1-IkBa construct. The cDNA encoding human IkBa (from aas 38-280) was cloned into the Bgl II and EcoRI sites of the MIGR1 retroviral plasmid [36, 37]. The expression of IkBa was confirmed by western blot analysis of transfected 293 cell line (data not shown).Back to article page