Figure 4
From: NF-kB inhibitor blocks B cell development at two checkpoints
Bcl-X completely rescues the Pro-B to Pre-B transition in IkBα-transduced chimeras Bone marrows derived from Bcl-X transgenic (WTtg) and non-transgenic (WT) mice were infected with MIGR1 control and IkBα-viruses in vitro and transferred to lethally-irradiated recipients as described in Materials and Methods. Two and four weeks after bone marrow transplantation, reconstituted bone marrow cells were isolated from the MIGR1 control and IkBα-transduced chimeras. Cells were stained with biotin-conjugated B220 (A) or co-stained with PE-conjugated CD43 and biotin-conjugated B220 antibodies (B) followed by flow cytometry. For (B), the percentage of cells expressing B220 and CD43 was determined based on the GFP+ population.