Figure 3

IkBα overexpression impairs the maturation of follicular and marginal zone B cells in the spleen Purified splenic B cells (>95% B220+) were isolated from the MIGR1 control and IkBα-transduced chimeras two and four weeks after bone marrow transplantation. (A) Cells were co-stained with biotin-conjugated CD21 and PE-conjugated CD23 antibodies and analyzed by flow cytometry. After gating on GFP+ population, the percentage of follicular B cells (CD21+/CD23+), marginal zone B cells (CD21+/CD23-), and transitional B cells (CD21-/CD23-) were enumerated. (B) Cells were co-stained with biotin-conjugated CD24 and PE-conjugated IgM antibodies and analyzed by flow cytometry. After gating on GFP+ population, the percentage of Transitional immature B cells (CD24-hi, IgM+) and mature B cells (CD24-lo, IgM+) were enumerated. (C) Cells were co-stained with biotin-conjugated CD21 and PE-conjugated IgM antibodies and analyzed by flow cytometry. After gating on the GFP+ population, the percentage of Transitional 1 B cells (CD21-, IgM+) and more mature B cells (CD21+, IgM+, containing T2, FB, and MZ B cells) were enumerated.