Figure 1
From: NF-kB inhibitor blocks B cell development at two checkpoints
Transfection efficiency, viral titer, and infection efficiency Three plasmids (pHIT123, pCGP and MIGR1 or MIGR1-IkB) were transfected into 293T cell for 48 hours. The supernatant was collected and tested for viral titer by using NIH3T3 cell line. The viral supernatant was subsequently used for infecting bone marrow cells. Flow cytometry was performed to determine the transfection efficiency on 293T cells (A), viral titer on 3T3 cells (B), and viral infection efficiency on bone marrow cells (C) prior to transfer into lethally-irradiated recipient mice.