Figure 2

IkBα overexpression leads to a reduction in the Pre-B/immature B cell compartment in the bone marrow Bone marrow cells were isolated from the MIGR1 control and IkBα-transduced chimeras at two and four weeks post-BM transplantation. Cells were stained with biotin-conjugated B220 (A) or co-stained with PE-conjugated CD43 and biotin-conjugated B220 antibodies (B) and analyzed by flow cytometry. For (B), B220+ and CD43+ populations were assessed based on the GFP+ population. The data is a representative of five experiments.